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. 2019 Mar 14;9:4548. doi: 10.1038/s41598-019-39973-x

Figure 3.

Figure 3

PFO-D4–accessible cholesterol in the outer leaflet of the PM is increased by ABCA1. (A) PFO-D4–accessible cholesterol in the outer leaflet of the PM was analyzed by flow cytometry. PFO-D4 was labeled with Alexa Fluor 647. FreeStyle293-F cells with fluorescent intensities greater than 20,000 were defined as GFP-positive, and others as GFP-negative. Linear regression between GFP and Alexa Fluor 647 is shown as a black line. (B) Fold change in the median value of Alexa Fluor 647 fluorescence intensity in GFP-positive vs. GFP-negative cells.