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. 2019 Mar 14;9:4548. doi: 10.1038/s41598-019-39973-x

Figure 6.

Figure 6

Cholesterol-transporting ABC proteins inhibit SLO pore formation. (A) HEK293 cells transiently transfected with plasmids encoding GFP, ABCA1-GFP, ABCA7-GFP, ABCB1-GFP, or ABCG1 were treated with SLO and DAPI (original images are shown in Fig. S3). (B) DAPI-stained and -unstained GFP-positive cells were counted, and proportions were calculated. Average values are shown with S.E. For each sample, images were acquired at five positions in the dish. **P < 0.001 compared with control (GFP). n.s. P > 0.05 compared with control (GFP). (C) Cholesterol content in the outer leaflet of the PM was analyzed by FACS with PFO-D4 labeled with Alexa Fluor 647. FreeStyle293-F cells with fluorescence intensity greater than 50,000 were defined as GFP-positive, and others as GFP-negative. Original data are shown in Supplementary Fig. 3.