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. 2019 Mar 14;9:4476. doi: 10.1038/s41598-019-40940-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Annotated mitochondrial sequence indicating gene position and G/C richness regions. Outer circle represents aligned genes with outermost representing the heavy strand (H) and adjacent inner ring the light strand (L). Green represents protein coding regions, pink tRNA, blue rRNA, and grey is the D-loop. Middle circle in black represents GC content: Protruding outwards indicates GC rich while inward protrusion shows AT rich regions. The inner most circle is GC skew (G − C/G + C) with positive skew (between 0 and 1) represented by green and negative as purple (between 0 and −1).