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. 2019 Mar 14;9:4599. doi: 10.1038/s41598-018-37622-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA and protein expression of KIF4A, 9, 18A, and 23 in LGG and GBM. (A) The relative mRNA expression of KIF4A, 9, 18A, and 23 was compared among the normal control, LGG, and GBM (upper). The mRNA expression of KIF4A, 9, 18A, and 23 in LGG and GBM merged dataset was compared with that in normal samples in the TCGA dataset (lower). The fold change values of KIF4A, 9, 18A, and 23 were 11.9, 0.9, 8.8, and 6.6, respectively (lower left). The relative mRNA expression of KIF4A, 9, 18A, and 23 was compared in LGG and GBM merged dataset (lower right). (B) Representative IHC images of KIF4A, 9, 18A, and 23 in normal cerebrum, low-grade glioma, and high-grade glioma in Human Protein Atlas. Image available from v18.proteinatlas.org^18–20. The scale bar indicates 50 µm. (C) Comparison of the mRNA expression of KIF4A, 9, 18A, and 23 in LGG between histologic grades 2 and 3. The distribution was compared between the two groups by t-test. A p-value < 0.05 was deemed to indicate statistical significance. *p < 0.05, **p < 0.01, ***p < 0.001.