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. 2019 Mar 14;9:4536. doi: 10.1038/s41598-019-40809-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Wnt signaling regulates the growth of gastric organoids in air-liquid interface. The growth of organoids were examined using stereoscope (a) and Ki67 staining (b) at Day 21 after treating with 10 uM LGK974 for 7 days. 0.1% DMSO in organoid growth media as the vehicle control. (c) Differentially expressed Wnt related genes between epithelial and fibroblast cells. Genes with fold change >1.5 (|Ln| > 0.4) and Bonferoni adjusted p < 0.05 were labeled in red with gene name. The x-axis is the natural log transformed fold change of gene expression in epithelial vs mesenchymal cells. The y-axis is the −log10 transformed p value.