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. 2019 Mar 14;9:4494. doi: 10.1038/s41598-019-40960-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of ZIKV in virus-spiked mosquito samples and crude lysate of experimentally infected Aedes aegypti. Crude lysates of uninfected A. aegypti were spiked with ZIKV to result in either a high (1 × 10⁶ PFU/mL) or low viral load (1 × 10³ PFU/mL) and processed for RT-LAMP without RNA isolation (A–C). (D–F) Represents RT-LAMP results of experimentally infected mosquitoes. RT-LAMP amplicons were observed by visual color change of the products and gel electrophoresis. The amplification products were observed by naked eye under natural light (A,D), under UV irradiation (B,E) and agarose gel electrophoresis (C,F). Legends in (A–C) are: NTC (non-template control): water; (−): macerate of uninfected Aedes aegypti; (10⁶): macerate of Aedes aegypti spiked with 10⁶ PFU; (10³): macerate of Aedes aegypti spiked with 10³ PFU. Legends in (D–F) are: NTC (non-template control): water; (−): macerate of uninfected Aedes aegypti; (+):macerate of Aedes aegypti experimentally infected with ZIKV. M: molecular weight marker.