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. 2019 Mar 14;9:4494. doi: 10.1038/s41598-019-40960-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analytical specificity of ZIKV RT-LAMP in mosquito samples. Crude lysates of uninfected A. aegypti were spiked with different arboviruses (DENV 1–4, YFV, CHIKV and ZIKV) circulating in Brazil so the final concentration would be 10⁶ PFU per reaction. Spiked samples were then assayed by ZIKV RT-LAMP. The amplification products were observed by naked eye under natural light (A), under UV irradiation (B) and agarose gel electrophoresis (C). M: molecular weight marker. NTC (non-template control): water.