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. 2019 Mar 14;9:4494. doi: 10.1038/s41598-019-40960-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ZIKV detection by RT-LAMP in mosquito samples at the qRT-PCR detection limit. Mosquito samples at the detection threshold by qRT-PCR (Ct values ranging from 37.5 to 40.3) were assayed by RT-LAMP and all reactions showed a positive result. The amplification products were observed by naked eye under natural light (A), under UV irradiation (B) and agarose gel electrophoresis (C). M: molecular weight marker. NTC (non-template control): water.