Table 2.

Detection limit of the ZIKV RT-LAMP assay^a.

ZIKV Concentration (PFU)	No. of Replicates	No. of positive results	Hit rate in %
105	10	10	100
104	10	10	100
103	10	10	100
102	10	10	100
101	10	10	100
100	10	10	100
10−1	10	10	100
10−2	10	10	100
10−3	10	9	90
10−4	10	7	70
10−5	10	6	60
10−6	10	0	0
10−7	10	0	0

^aProbit regression analysis was calculated using MedCalc software (version 18.11), giving a C₉₅ value (concentration detectable 95% of the time) of −2,98 log₁₀ PFU of ZIKV. This indicates that the limit of detection is about −3 log₁₀ (1/1000) PFU/reaction and that samples containing that concentration would be detected 95% of the time.