Table 3.

Mosquito samples used for RT-LAMP validation.

Sample (ID)	Ct value	ZIKV PFU/mL equivalent	Mosquito sample	Source	Result of RT-LAMP
1	27.0	6.20 × 106	Culex quinquefasciatus	Field sample	+
2	29.0	3.97 × 106	Culex quinquefasciatus	Field sample	+
3	29.0	3.97 × 106	Aedes aegypti	Laboratory sample	+
4	30.0	1.50 × 106	Culex quinquefasciatus	Field sample	+
5	30.0	1.50 × 106	Culex quinquefasciatus	Field sample	+
6	30.0	1.50 × 106	Aedes aegypti	Laboratory sample	+
7	30.5	5.31 × 106	Aedes aegypti	Field sample	+
8	30.6	1.45 × 106	Aedes aegypti	Laboratory sample	+
9	30.6	1.45 × 106	Aedes aegypti	Laboratory sample	+
10	31.0	8.23 × 106	Aedes aegypti	Field sample	+
11	31.0	8.23 × 106	Aedes aegypti	Field sample	+
12	31.0	8.23 × 106	Culex quinquefasciatus	Field sample	+
13	31.0	8.23 × 106	Aedes aegypti	Laboratory sample	+
14	32.0	3.91 × 105	Aedes aegypti	Field sample	+
15	32.0	3.91 × 105	Aedes aegypti	Field sample	+
16	32.0	3.91 × 105	Culex quinquefasciatus	Field sample	+
17	32.0	3.91 × 105	Culex quinquefasciatus	Field sample	+
18	33.0	2.27 × 105	Culex quinquefasciatus	Field sample	+
19	34.0	9.97 × 105	Aedes aegypti	Field sample	+
20	34.0	9.97 × 105	Aedes aegypti	Field sample	+
21	34.5	5.17 × 105	Aedes aegypti	Field sample	+
22	35.3	3.23 × 104	Aedes aegypti	Field sample	+
23	35.5	3.00 × 104	Aedes aegypti	Field sample	+
24	35.5	3.00 × 104	Aedes aegypti	Field sample	+
25	36.5	1.41 × 104	Aedes aegypti	Field sample	+
26	36.5	1.41 × 104	Aedes aegypti	Field sample	+
27	37.5	6.00 × 101	Culex quinquefasciatus	Field sample	+
28	38.0	5.60 × 101	Aedes aegypti	Field sample	+
29	38.0	5.60 × 101	Aedes aegypti	Field sample	+
30	38.6	5.00 × 101	Aedes aegypti	Field sample	+
31	39.0	4.15 × 101	Culex quinquefasciatus	Field sample	+
32	40.3	0	Culex quinquefasciatus	Field sample	+
33	>40.0	0	Aedes aegypti	Field sample	−
34	>40.0	0	Aedes aegypti	Field sample	−
35	>40.0	0	Aedes aegypti	Field sample	−
36	>40.0	0	Aedes aegypti	Field sample	−
37	>40.0	0	Aedes aegypti	Field sample	−
38	>40.0	0	Culex quinquefasciatus	Field sample	−
39	>40.0	0	Culex quinquefasciatus	Field sample	−
40	>40.0	0	Culex quinquefasciatus	Field sample	−
41	>40.0	0	Culex quinquefasciatus	Field sample	−
42	>40.0	0	Culex quinquefasciatus	Field sample	−
43	>40.0	0	Culex quinquefasciatus	Field sample	−
44	>40.0	0	Culex quinquefasciatus	Field sample	−
45	>40.0	0	Culex quinquefasciatus	Field sample	−
46	>40.0	0	Culex quinquefasciatus	Field sample	−
47	>40.0	0	Culex quinquefasciatus	Field sample	−
48	>40.0	0	Culex quinquefasciatus	Field sample	−
49	>40.0	0	Culex quinquefasciatus	Field sample	−
50	>40.0	0	Culex quinquefasciatus	Field sample	−
51	>40.0	0	Culex quinquefasciatus	Field sample	−
52	>40.0	0	Culex quinquefasciatus	Field sample	−
53	>40.0	0	Culex quinquefasciatus	Field sample	−
54	>40.0	0	Culex quinquefasciatus	Field sample	−
55	>40.0	0	Culex quinquefasciatus	Field sample	−
56	>40.0	0	Aedes aegypti	Laboratory sample	−
57	>40.0	0	Aedes aegypti	Laboratory sample	−
58	>40.0	0	Aedes aegypti	Laboratory sample	−
59	>40.0	0	Aedes aegypti	Laboratory sample	−
60	>40.0	0	Aedes aegypti	Laboratory sample	−