Table 1.
Strain data, MICs and molecular resistance mechanisms.
| Isolate | Sex | Age | Source | ß-lactams | Chloramphenicol | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AMP | AMC | CXM | CRO | CTX | FEP | IPM | MEM | PBP3 | CHL | ||||||
| HUB11505 | M | 25 | Urethral | 4 | 2/1 | >8 | ≤0,12 | 0,5 | 0.5 | 0,25 | ≤0,25 | S385T, I442F, V511A, N526K, V562I | >8 | catS | |
| HUB12345 a | F | 38 | Vaginal | >4 | >4/2 | >8 | 0,25 | 1 | 2 | 0,5 | ≤0,25 | K276N, A307N, V329I, S385T, I442F, V511A, N526K, V562I | >8 | catS | |
| HUB12445 a | F | 38 | Endocervical | >4 | >4/2 | >8 | 0,25 | 1 | 2 | 0,5 | ≤0,25 | K276N, A307N, V329I, S385T, I442F, V511A, N526K, V562I | >8 | catS | |
| HUB 12640 | M | 35 | Preputial | 4 | 4/2 | >8 | 0,25 | 1 | 2 | 0,5 | ≤0,25 | K276N, A307N, V329I, S385T, I442F, V511A, N526K, V562I | >8 | catS | |
| Isolate | Macrolides | Quinolones | Tetracycline | Cotrimoxazole | Rifampicin | ||||||||||
| ERY | AZM | L22 | MEGA | CIP | LVX | GyrA | ParC | ParE | TET | SXT | DHFR | RIF | |||
| HUB11505 | >16 | >4 | A69S | mef(E) | >2 | >4 | S84F, D88Y | S84F, S138T, M198L | D420N, A451S | >4 | tet(M) | tet(B) | >2/38 | 1 | |
| HUB12345 a | >16 | >4 | A69S | mef(E) | >2 | >4 | S84F, D88Y | S84F, S138T, M198L | D420N, A451S | >4 | tet(M) | >2/38 | I95L | 0.5 | |
| HUB12445 a | >16 | >4 | A69S | mef(E) | >2 | >4 | S84F, D88Y | S84F, S138T, M198L | D420N, A451S | >4 | tet(M) | >2/38 | I95L | 0.5 | |
| HUB12640 | >16 | >4 | A69S | mef(E) | >2 | >4 | S84F, D88Y | S84F, S138T, M198L | D420N, A451S | >4 | tet(M) | tet(B) | >2/38 | 0.5 | |
MICs for each antibiotic are expressed in mg/L. Resistance based on the EUCAST criteria for H. influenzae is marked in bold. Resistance genotype refers to amino acid changes and acquired resistance mechanisms for ß-lactams, macrolides, quinolones, tetracycline and co-trimoxazole.
AMP, ampicillin; AMC, amoxicillin/clavulanic acid; CXM, cefuroxime; CRO, ceftriaxone; CTX, cefotaxime; FEP, cefepime; IPM, imipenem; MEM, meropenem; CHL, chloramphenicol; ERY, erythromycin; AZM, azithromycin; CIP, ciprofloxacin; LVX, levofloxacin; TET, tetracycline; SXT, co-trimoxazole; RIF, rifampicin.
aIndicates isolates from the same patient obtained on the same day.