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. 2019 Mar 14;9:4553. doi: 10.1038/s41598-019-41074-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Summary receiver operating characteristic (SROC) curves for PCR. Each individual study is represented by an empty circle. The size of the circle is proportional to the sample size of the study. The solid circles correspond to the summary estimates of sensitivity and specificity and are shown with 95% confidence regions (dotted lines) and 95% prediction regions (dashed lines).