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. 2019 Mar 14;9:4531. doi: 10.1038/s41598-018-36060-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mitochondrial coupling and ATP content measured in thorax muscle of Drosophila melanogaster males exposed to either a standard diet (SD) or a high-fat diet (HFD). (A) P/L ratio at the level of complex I (P/L = CI-OXPHOS/CI-LEAK), as an indicator of mitochondrial quality and of mitochondrial coupling (N = 6), (B) ATP content (N = 3). Results are means ± s.e.m for each day of the exposure and for each diet, and have been analyzed using a two-way ANOVA followed by pairwise comparisons of the least-squares means using adjusted P-values (Tukey method). Stars depict significant differences between the SD and the HFD on each day of exposure with: *P < 0.05, **P < 0.01, ***P < 0.001. Dissimilar lowercase letters denote changes between days of exposure to a specific diet, with each different letter(s) representing statistically significant differences (P < 0.05).