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. 2019 Mar 14;9:4541. doi: 10.1038/s41598-019-40866-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cancer cell proliferations were suppressed after 48-h drug treatment in the zebrafish xenotransplanted with human A549 non-small-cell lung cancer cells, human SGC-7901 stomach cancer cells, human HepG2 liver cancer cells, human JF 305 pancreatic cancer cells, and human MCF-7 breast cancer cells. a1, b1, c1, d1 and e1 were the control xenotransplanted zebrafish without drug treatment. a2–a6 were treated with Bevacizumab, cis-Platinum, Endostar, Paclitaxel and Vinorelbine; b2–b6 were treated with 5-FU, Hydroxyurea, cis-Platinum, Irinotecan and Paclitaxel; c2–c6 were treated with Adriamycin, Gemcitabine, Hydroxyurea, cis-Platinum and 5-FU; and d2–d4 as well as e2–e4 were treated with Furanodiene at concentrations of 1/9 MTC, 1/3 MTC and MTC, respectively. A representative entire body of the xenotransplanted zebrafish body was shown in f.