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. 2019 Mar 14;9:4421. doi: 10.1038/s41598-019-38806-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression profile of the Ae. aegypti and An. gambiae 5-HT₂ receptor family. Quantitative RT-PCR was conducted on the immature and mature life stages with SYBRGreen for the 5-HT2 genes. The expression level of the genes was determined via comparative quantification. Housekeeping gene, 40S ribosomal protein S7 (AAEL009496 and AGAP010592) was utilized as internal controls. Fold change ± SEM compared to the male whole body is presented. Two independent experiments were each performed with three technical replicates.