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. 2019 Mar 14;9:4421. doi: 10.1038/s41598-019-38806-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Locomotion and the serotonergic system in Ae. aegypti. (a) Flight success after microinjection. (b) Flight ability after treatment with methiothepin, a 5-HT receptor antagonist. (c) Flight success after treatment with methiothepin and 5-HT. (d) Flight ability post microinjection with fluoxetine, a selective serotonin-reuptake inhibitor. Three independent trials were conducted. Box plots represent the median value (line), mean value (+), interquartile range (box), and Tukey whiskers equivalent to 1.5 fold of the interquartile range. A distance of 91.5 cm was recorded for each mosquito that did not fly. ^•Represents an individual mosquito outside the range of the Tukey whiskers. Data were compared with the Kruskal-Wallis and Dunnett’s multiple comparison tests after failing the Kolmogorov-Smirnov normality test. **p-value < 0.002; ***p-value < 0.0002.