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. 2019 Mar 14;9:4602. doi: 10.1038/s41598-019-41045-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Similarity plot generated in SimPlot using the canine astrovirus sequence (DF-BC15- CAV-AUS-2017) from the dog samples as the query sequence against seven other reference sequences from NCBI Genbank. Divergence between DF-BC15-CAV-AUS-2017 and each reference sequence over the 6612 nucleotides genome using a 200 nucleotides sliding window at 20 nucleotides intervals and the F84 distance⁴⁶ model with the maximum likelihood method. Percentage identities at each analysis point were plotted on a line chart. For similarity plot analysis, the y-axis shows the percentage similarity between the parental sequences and the query sequence. Different colors represent a different reference sequence.