Table 1.
Antibodies used for immunohistochemistry.
| Antigen, Target | Company; product number; clone | Blocking Serum | Pre-treatment | Dilution | 2nd antibody (1:200) |
|---|---|---|---|---|---|
| TMEV | — | Goat | — | 1:2000 | Goat anti-rabbit |
| LivingColors DsRed, TdTomato | Takara Bio Europe/Clontech 632496 | Goat | Microwave, 20 minutes, citrate buffer | 1:500 | Goat anti-rabbit |
| β-APP, axonal damage |
Merck/Millipore MAB348 | Goat | Microwave, 20 minutes, citrate buffer | 1:2000 | Goat anti-mouse |
| Periaxin, Schwann cells |
Sigma HPA001868 | Goat | Microwave, 20 minutes, citrate buffer | 1:5000 | Goat anti-rabbit |
| CD3, T lymphocytes |
Dako/Agilent Technologies A0452 | Goat | Microwave, 20 minutes, citrate buffer | 1:250 | Goat anti-rabbit |
| CD45R, B lymphocytes |
BD Bioscience 553085 | Goat | Microwave, 20 minutes, citrate buffer | 1:1000 | — |
| CD107b, microglia/macrophages |
Bio Rad MCA2293 | Goat | Microwave, 20 minutes, citrate buffer | 1:400 | Goat anti-rat |
| MBP, myelin |
Chemicon AB980 | Goat | — | 1:500 | Goat anti-rabbit |
TMEV = Theiler’s murine encephalomyelitis virus; β-APP = beta-amyloid-precursor protein; MBP = myelin basic protein.