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. 2018 Jun 4;10(4):300–305. doi: 10.1007/s13238-018-0554-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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TLE4 suppresses production of IL-6 and IL-12 and protects host from septic shock. (A) Quantitative real-time PCR analysis of Tle4 mRNA in BMDMs obtained from littermates of Tle4^+/+, Tle4^+/− and Tle4^−/− mice. ND, not detected. (B) Quantitative real-time PCR analysis of Il6 and Il12b mRNA in Tle4^+/+, Tle4^+/− and Tle4^−/− BMDMs with or without LPS stimulation. (C) Cumulative results of Il6 and Il12b mRNA in Tle4^+/+ and Tle4^−/− BMDMs stimulated with LPS for 3 h, presented relative to that in Tle4^+/+ BMDMs. (D) ELISA of IL-6 and IL-12p40 in supernatants from Tle4^+/+ and Tle4^−/− BMDMs stimulated for the indicated periods with LPS. (E) Quantitative real-time PCR of Tle4 mRNA in BMDMs from Lyz2-Cre and Tle4^fl/fl Lyz2-Cre (TLE4 cKO) mice. (F) Quantitative real-time PCR of Il6 and Il12b mRNA in Lyz2-Cre and TLE4 cKO BMDMs with or without LPS stimulation. (G) Cumulative results of Il6 and Il12b mRNA in Lyz2-Cre and TLE4 cKO BMDMs stimulated with LPS for 3 h, presented relative to that in Lyz2-Cre BMDMs. (H) ELISA of IL-6 and IL-12p40 in supernatants of BMDMs from Lyz2-Cre and TLE4 cKO mice stimulated for the indicated periods with LPS. (I and J) Survival rate (I) and body weight (J) of Lyz2-Cre and TLE4 cKO mice injected intraperitoneally with 15 mg/kg of LPS. Data are representative of four (A, B and D) or six (E, F and H) independent experiments (mean ± s.d. of technical triplicates in A, B, E and F), or are pooled from four (C), six (G), two (I), or three (J) independent experiments. *P < 0.05, **P < 0.01, ****P < 0.0001 (Student’s paired (C and G) or unpaired (J) t-test)