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. 2019 Mar 8;6:18. doi: 10.3389/fnut.2019.00018

Figure 1.

Figure 1

Artemisia scoparia extracts, fractions, and subfractions progeny. Shaded fractions and subfractions were active. For crude fractions: light shading indicates significant enhancement of lipid accumulation as measured by ORO staining, while dark shading indicates FC > 1.5 in ORO staining vs. controls. For HPLC fractions of EA, light shading indicates significant effects on ORO staining and adipogenic gene expression at 20 μg/ml dose; dark shading indicates significant effects on ORO staining and gene expression at 5 and 20 μg/ml doses. For FCPC fractions of EA, light shading indicates significant effects on ORO staining at both doses tested (2 and 10 μg/ml); dark shading indicates fractions that enhanced ORO staining by 2-fold or greater. For subfractions of EA-F2, light shading indicates a significant effect on ORO staining at any dose and FC < 1.5, while dark shading indicates fractions that enhanced ORO staining by 1.5-fold or more at any dose. For subfractions of F2-2, shading indicates significant increases in ORO staining and in AdipoQ and Fabp4 gene expression at both doses tested.