Figure 4.

Adipogenesis in 3T3-L1 cells is enhanced by HPLC subfractions of EA. Cells were induced to differentiate using half-strength MDI cocktail containing DMSO vehicle, 20 μg/ml of EA, or 1, 5, or 20 μg/ml of each HPLC subfraction of EA. Four days after induction, cells were fixed and stained with Oil Red O (A); absorbance was measured in isopropanol eluates to quantitate staining (B). Cells were also harvested for RNA isolation and reverse transcription, expression of adiponectin (AdipoQ), aP2 (Fabp4), and FAS (Fasn) were assayed by qPCR (C). All data expressed as fold-change vs. DMSO vehicle controls. ^*p < 0.05,^§ p < 0.01, ^†p < 0.001, ^‡p < 0.0001 vs. DMSO controls.