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. 2019 Mar 8;6:18. doi: 10.3389/fnut.2019.00018

Figure 6.

Figure 6

3,5-DCQA does not enhance adipogenesis in 3T3-L1 cells. Cells were induced to differentiate using half-strength MDI cocktail containing DMSO vehicle, 50 μg/ml SCO, or 1, 5, or 20 μg/ml of 3, 5-dicaffeoylquinic acid (DCQA). (A) 4 days after induction, cells were fixed and stained with Oil Red O. Staining was quantitated by measuring absorbance in isopropanol eluates. (B) 3 days after induction, cells were harvested for RNA purification and reverse transcription; relative expression of AdipoQ, Fabp4, and Fasn were assayed by qPCR. All data expressed as fold-change vs. DMSO vehicle controls. †p < 0.001, ‡p < 0.0001 vs. DMSO controls. Three independent experiments were performed with the same results.