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. 2019 Mar 14;9:4560. doi: 10.1038/s41598-019-41029-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Formation of a pre-assembly compartment (PrAC) containing Golgi stacks and LC3 starts early in the viral life cycle. (A,B) Representative images of HFF infected with HCMV AD169 strain at MOI 1 during 10 to 48 hours and immunostained for IEA (immediate-early viral antigens) and for GM130 (A) or LC3 (B). Nuclei were subsequently stained with DAPI. Representative images of 2 independent experiments. Scale bars = 10 µm.