Skip to main content
. 2019 Mar 8;10:266. doi: 10.3389/fpls.2019.00266

FIGURE 4.

FIGURE 4

(A) Protein blot of crude extract from untransformed (WT Gamtoos-R) and wheat transformed with pUBI-OTS1 or empty vector (pUBI) prior to (day 0) and after exposure (day 7 and 14) to water stress, probed with anti-Rubisco (LSU) and (SSU) IgG. All lanes were loaded with 20 μg total protein. Blots were probed with a 1:7 000 dilution of the polyclonal IgG against LSU, SSU. The leaf proteins were resolved by 12% (w/v) sodium dodecyl sulfate–polyacrylamide gel electrophoresis prior to transferring to nitrocellulose. Images were cropped for presentation purposes. T1–T3 represents three independent transgenic events. (B) Gel densitometric analysis of the protein blot in (A) of the rubisco large (LSU, 54 kDa) and small (SSU, 14 kDa) subunits in the leaf crude protein extracts from untransformed (WT Gamtoos-R) and wheat transformed with pUBI-OTS1 or empty vector (pUBI) prior to (day 0) and after exposure (day 7 and 14) to water stress. Data are expressed as relative levels of rubisco protein compared with the basic level in control line (mean value of 1.0). Each bar is the mean of three independent values (biological replicates) ± SE.