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. 2019 Mar 8;10:410. doi: 10.3389/fmicb.2019.00410

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Copyright © 2019 Cao, Song, Li, Li, Lin, Chen, Chen, Xu, Ding and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic diagram of the assembled shuttle vectors in S. oneidensis MR-1. The cutting sites of the restriction enzymes are shown in red. Sa, SacI; E, EcoRI; Xb, XbaI; A, AvrII; Nd, NdeI; Nh, NheI; Xh, XhoI; Sp, SpeI; P, PstI; K, KpnI; H, HindIII. High-strength promoters (i.e., pBAD, pXyl, pCI, pJ23119, and pTac) are shown in orange; medium-strength promoters (pTet and pTrc^∗) are shown in aqua; and low-strength promoters (placUV5 and pLlacO1) are shown in olive.