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. 2019 Mar 8;10:410. doi: 10.3389/fmicb.2019.00410

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Copyright © 2019 Cao, Song, Li, Li, Lin, Chen, Chen, Xu, Ding and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of the inducible promoters by regulating inducer concentration and induction time in S. oneidensis MR-1. (A–F) GFP expression under the control of inducible promoters was measured at 6, 10, 24, 32, and 48 h after induction with a series of different inducer concentrations. (A–C) IPTG-inducible pTac, pLlacO1, and placUV5; (D) arabinose-inducible pBAD; (E) xylose-inducible pXyl; (F) aTc-inducible pTet). (G) GFP expression was measured at 24 h after induction with different inducer concentrations. The error bars (mean ± SD) were derived from triplicate experiments for each strain.