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. 2019 Mar 8;10:410. doi: 10.3389/fmicb.2019.00410

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Copyright © 2019 Cao, Song, Li, Li, Lin, Chen, Chen, Xu, Ding and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of replication origins in S. oneidensis MR-1. (A) Replicon copy numbers of different replication origins in S. oneidensis MR-1 determined by RT-qPCR. (B) GFP expression of various replication origins in S. oneidensis MR-1. S. oneidensis MR-1 harbored a single plasmid carrying pBBR1, p15A, pSC101, or ColE replication origin. In each case, the gfp gene was expressed from the same promoter, pTrc^∗ (no repressor lacO). The error bars (mean ± SD) were derived from triplicate experiments for each strain.