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. 2019 Mar 8;10:410. doi: 10.3389/fmicb.2019.00410

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Copyright © 2019 Cao, Song, Li, Li, Lin, Chen, Chen, Xu, Ding and Song.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fine-tuning MtrCAB expression to affect EET efficiency determined by WO₃ reduction assay. (A) Color development of the WO₃ reduction assay. Culture medium without bacteria was used as a control to eliminate disturbances caused by abiotic factors. The mtrCAB gene was overexpressed in the S. oneidensis ΔmtrA strain under the control of promoters placUV5, pTet, and pBAD, which were named as S-ΔmtrA, L-mtrCAB, M-mtrCAB, and H-mtrCAB, respectively, according to their level of expression (L, low; M, medium; H, high). (B) ‘Density (mean)’ analysis of each strain from the WO₃ reduction assay using ImageJ Software V.1.8.0. The error bars (mean ± SD) were derived from triplicate experiments for each strain.