Table 1.
Strains and plasmids used in the study.
| Strain | Description | Source |
|---|---|---|
| E. coli MG1655 | Wild type | Lab stock |
| S. oneidensis MR-1 | Wild type | Lab stock |
| E. coli WM3064 | A DPA auxotroph of E. coli could transfer plasmid into S. oneidensis MR-1 by conjugation | Lab stock |
| E. coli Trans1 T1 | Cloning strain | TransGen |
| E. coli BL21(DE3) | Cloning strain | TransGen |
| S-ΔmtrA | A mtrA deletion mutant of S. oneidensis MR-1 | Lab stock |
| L-mtrCAB | S-ΔmtrA carrying pHG13-placUV5-CoIE-MtrCAB | This study |
| M-mtrCAB | S-ΔmtrA carrying pHG13-pTet-CoIE-MtrCAB | This study |
| H-mtrCAB | S-ΔmtrA carrying pHG13-pBAD-CoIE-MtrCAB | This study |
| Plasmid | Description | Source |
| pHG11 | reppBBR1, KanR without PstI restriction site, Mob without BsaI restriction site | Lab stock |
| pHG12 | reppBBR1, KanR without PstI and NcoI restriction sites, Mob without BsaI restriction site, with insertion of SacI in front of EcoRI restriction site. | This study |
| pHG12-pBAD | pBAD, reppBBR1, KanR, Mob | This study |
| pHG12-pXyl | pXyl, reppBBR1, KanR, Mob | This study |
| pHG12-pTet | pTet, reppBBR1, KanR, Mob | This study |
| pHG12-pTrc∗ | pTrc∗, reppBBR1, KanR, Mob | This study |
| pHG12-pTac | pTac, reppBBR1, KanR, Mob | This study |
| pHG12-pLlacO1 | pLlacO1, reppBBR1, KanR, Mob | This study |
| pHG12-placUV5 | placUV5, reppBBR1, KanR, Mob | This study |
| pHG12-pCI | pCI, reppBBR1, KanR, Mob | This study |
| pHG12-pJ23119 | pJ23119, reppBBR1, KanR, Mob | This study |
| pHG12-pBAD-GFP | pBAD, reppBBR1, KanR, Mob, gfp amplified from pLac-GFP | This study |
| pHG12-pXyl-GFP | pXyl, reppBBR1, KanR, Mob, gfp | This study |
| pHG12-pTet-GFP | pTet, reppBBR1, KanR, Mob, gfp | This study |
| pHG12-pTrc∗-GFP | pTrc∗, reppBBR1, KanR, Mob, gfp | This study |
| pHG12-pTac-GFP | pTac, reppBBR1, KanR, Mob, gfp | This study |
| pHG12-pLlacO1-GFP | pLlacO1, reppBBR1, KanR, Mob, gfp | This study |
| pHG12-placUV5-GFP | placUV5, reppBBR1, KanR, Mob, gfp | This study |
| pHG12-pCI-GFP | pCI, reppBBR1, KanR, Mob, gfp | This study |
| pHG12-pJ23119-GFP | pJ23119, reppBBR1, KanR, Mob, gfp | This study |
| pHG13-pTrc∗-CoIE-GFP | pTrc∗, repColE, CmR, gfp, oriT | This study |
| pHG13-pTrc∗-p15A-GFP | pTrc∗, repp15A, CmR, gfp, oriT | This study |
| pHG13-pTrc∗-pSC101-GFP | pTrc∗, reppSC101, CmR, gfp, oriT | This study |
| pHG13-pTrc∗-CoIE-BFP | pTrc∗, repColE, CmR, oriT, BFP | This study |
| pHG13-pTrc∗-CoIE-CFP | pTrc∗, repColE, CmR, oriT, CFP | This study |
| pHG13-pBAD-CoIE-BFP | pBAD, repColE, CmR, oriT, BFP | This study |
| pHG13-pBAD-CoIE-CFP | pBAD repColE, CmR, oriT, CFP | This study |
| pHG13-pTet-CoIE-BFP | pTet, repColE, CmR, oriT, BFP | This study |
| pHG13-placUV5-CoIE-BFP | placUV5, repColE, CmR, oriT, BFP | This study |
| pHG13-pBAD-CoIE- MtrCAB | pBAD, repColE, CmR, oriT, mtrA, mtrC, mtrB | This study |
| pHG13-pTet-CoIE-MtrCAB | pTet,repColE, CmR, oriT, mtrA, mtrC, mtrB | This study |
| pHG13-placUV5-CoIE- MtrCAB | placUV5, repColE, CmR, oriT, mtrA, mtrC, mtrB | This study |