Figure 1.

Relative sequence abundance of bacterial families across mine environments. Sequences were obtained by Illumina sequencing of 16S rRNA gene amplicons (A) in parent communities (n = 9) of mining wastewater at Mine 1 (M1) [Tailings reservoirs (0.5 m) in September and October], Mine 2 (M2) [Tailings reservoir (0.5 m) in September], Mine 3 (M3) [Tailings reservoir (2 m) in September, Input water, and Tailings Reservoir (0.5 m and 10 m) in November], and Mine 4 (M4) [Tailings reservoir (7 m) and Receiving Environment in September] and (B) the communities grown through sulfur oxidizing bacterial (SoxBac) enrichment experiments (n = 9). “Others” is defined as organisms that had lower relative abundance than the 10 bacterial families with the highest proportions across all samples.