Table 1.

Physico-and geochemical characterization of the different mining environments used for SoxBac enrichment experiments.

Field site	Depth (m)	pH	Temp (°C)	O2 (%)	Total SUF (mmol L−1)	Total S0.2μm (mmolL−1)	S2${\text{O}}_3^{2-}$ (mmol L−1)	$\text{S}{\text{O}}_3^{2-}$ (mmol L−1)	$\text{S}{\text{O}}_4^{2-}$ (mmol L−1)	Sreact (TotS-$\text{S}{\text{O}}_4^2$) (mmol L−1)	Sreact Proportion (%)
Mine 1 tailings reservoir (September)	0.5	6.0	–	–	2.3 ± 0.02	2.3 ± 0.01	<0.002	0.006 ± 0.002	2.2 ± 0.4	0.1	4.3
Mine 1 tailings reservoir (October)	0.5	6.96	–	–	2.3 ± 0.01	2.3 ± 0.01	<0.002	–	–	–	–
Mine 2 tailings reservoir (September)	0.5	6.9	9.9	87.7	6.9 ± < 0.01	7.1 ± 0.2	<0.002	0.018 ± 0.001	5.9 ± 0.1	1.0	14
Mine 3 tailings reservoir September	2	4.67	21.4	72.6	10.7 ± 0.2	10.7 ± 0.5	<0.002	0.008 ± < 0.001	6.9 ± 0.2	3.8	36
Mine 3 tailings reservoir (November)	0.5	6.94	11.3	30.6	9.5 ± 0.1	9.4 ± 0.1	–	–	8.3 ± < 0.01	1.2	13
Mine 3 tailings reservoir (November)	10	6.15	11.3	22	7.5 ± 0.04	7.5 ± 0.02	–	–	0.21 ± < 0.01	7.3	97
Mine 3 input water (November)	0.5	3.67	4.6	93.2	10 ± < 0.01	10 ± < 0.01	–	–	6.7 ± 0.1	3.3	33
Mine 4 tailings reservoir (September)	7	7.34	11.1	88.6	17.4 ± 0.9	16.9 ± 0.3	0.31 ± 0.03	0.009 ± < 0.001	11.8 ± 0.7	5.6	32
Mine 4 receiving environment (September)	0.5	4.33	11.0	39.1	14.9 ± 0.3	14.4 ± 0.4	<0.002	0.01 ± < 0.001	10.5 ± 0.6	4.4	30

Tailings reservoir; Mine 1, 2, 3, and 4; input waters (Mine 3) and receiving environments (Mine 4). Total S; TotS UF, unfiltered and filtered (0.2 μm), S₂O₃${}_{aq}^{2-}$, SO₃${}_{aq}^{2-}$, S${\text{O}}_4^{2-}$ and the reactive S concentration (S_react; defined as the difference between TotS and sulfate concentrations; values represent average and SD, n = 3).