. 2019 Mar 8;10:297. doi: 10.3389/fmicb.2019.00297

Table 2.

Initial and final sulfur speciation and pH in sulfur oxidizing microbial enrichments on their 3rd cycle grown from parent communities from the nine MIW samples.

	Cycle duration (days)	pH		S-S₂ $O_{3}^{2 -}$ (mmol L⁻¹)			S- $S O_{3}^{2 -}$ (mmol L⁻¹)			S- $S O_{4}^{2 -}$ (mmol L⁻¹)			S-unresolved (mmol L⁻¹)
		I	F	I	F	Δ%	I	F	Δ%	I	F	Δ %	I	F	Δ %
E Mine 1	5	7.1	6.1	19.9 ± 1.4	17.9 ± 0.2	−10	0.048 ± 0.01	0.39 ± 0.03	700	9.2 ± 0.6	12.4 ± 0.6	+34	26.9 ± 2.0	25.3 ± 2.0	−6
September
Tailings Reservoir
0.5 m
E Mine 1	3	7.0	6.7	19.8 ± 1.1	20.0 ± 0.5	+1	0.074 ± 0.046	0.03 ± 0.002	−59	4.3 ± 0.3	4.6 ± 0.7	+7	31.8 ± 2.7	31.4 ± 2.7	−1
October
Tailings Reservoir
0.5 m
E Mine 3	6	7.0	3.1	18.8 ± 1.4	0.1 ± 0.02	−99	0.024 ± 0.003	<0.01	−58	11.2 ± 0.6	14.4 ± 1.0	+29	30.0 ± 1.1	45.5 ± 1.1	+52
November
Tailings Reservoir
0.5 m
E Mine 3	6	7.0	3.0	19 ± 0.4	0.2 ± 0.05	−98	0.039 ± 0.007	<0.01	−74	12.0 ± 0.2	15.0 ± 0.8	+25	28.7 ± 1.1	44.8 ± 0.2	+156
November
Tailings Reservoir
10 m
E Mine 2	5	7.1	3.0	20.0 ± 1.7	0.1 ± 0.02	−99.5	0.03 ± 0.02	<0.01	−66	12.8 ± 0.1	23.3 ± 0.4	+82	22.3 ± 2.1	31.6 ± 2.1	+142
November
Input Water
0.5 m
E Mine 4	5	7.0	3.1	20.3 ± 0.9	0.1 ± 0.01	−99	0.07 ± 0.05	<0.01	−86	13.5 ± 0.3	22.5 ± 0.6	+67	25.1 ± 2.0	36.4 ± 2.0	+45
September
Tailings Reservoir
7 m
E Mine 4	5	7.0	3.9	19.6 ± 0.7	0.2 ± 0.04	−99	0.07 ± 0.04	<0.01	−86	11.3 ± 0.03	21.1 ± 0.6	+87	26 ±NA	35.7 ±NA	+37
September
Receiving Environment
0.5 m
E Mine 3	6	7.1	3.0	20 ± 1.7	0.1 ± 0.02	−99.5	0.04 ± 0.01	<0.01	−75	10.1 ± 0.3	17.5 ± 1.1	+73	30.7 ± 0.7	43.4 ± 0.7	+41
September
Tailings Reservoir
0.5 m
E Mine 3	7	7.0	3.1	19.7 ± 0.3	0.1 ± 0.05	−99.5	0.059 ± 0.05	<0.01	−83	8.3 ± 0.6	21.51 ± 0.6	+159	19 ±NA	25.3 ±NA	+33
September
Tailings Reservoir
2 m

Unresolved sulfur (S-unresolved) was determined by S mass balance subtracting the concentrations of all measured sulfur species from TotS_UF concentrations.