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. 2019 Feb 13;38(6):e99363. doi: 10.15252/embj.201899363

Figure 1. RsaI responds to glucose through the transcriptional factor CcpA.

Figure 1

  1. Northern blot experiments show the expression of RsaI during growth phase in the HG001 strain in MHB medium with or without the addition of 1.5 g/l of d‐glucose. Glucose was added either at the beginning of growth (+ glucose, left panel) or after 3 h of growth (right panel).
  2. Northern blot experiment shows the expression of RsaI during growth phase in the HG001, ∆ccpA mutant strain, and ∆codY mutant strain, in MHB medium with (+) or without (−) the addition of 1.5 g/l of d‐glucose.
  3. Northern blot experiment shows the expression of RsaI in the HG001 and ∆ccpA mutant strains. Total RNA was prepared after 2, 3, 4, 5, and 6 h of culture in BHI medium at 37°C.
  4. Northern blot analysis of RsaI in the HG001 strain grown in MHB medium with or without the addition of 1 g/l of glucose, fructose, or xylose. For all the experiments, loading controls were done using the expression of 5S rRNA (5S) as revealed after hybridization of the membranes with a specific probe. However for these controls, we used aliquots of the same RNA preparations, but the migration of the samples was performed in parallel to the experiments on a separate agarose gel because RsaI and 5S rRNA have very similar sizes.

Source data are available online for this figure.