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. 2019 Feb 13;38(6):e99363. doi: 10.15252/embj.201899363

Figure EV2. RsaI interacts with several RNA species.

Figure EV2

Gel retardation assays showing the formation of complexes between RsaI and several mRNAs, and the sRNA RsaD. The 5′ end‐labeled RsaI was incubated with increasing concentrations of target mRNAs or RsaD as shown on the top of the autoradiographies. Quantification of the autoradiographies showed that RsaI forms stable complexes with many of the mRNAs (Kd < 50 nM) except for TreB and RsaD (Kd > 200 nM).Source data are available online for this figure.