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. 2019 Feb 13;38(6):e99363. doi: 10.15252/embj.201899363

Figure 5. Regulatory function of RsaI is not impaired by binding of RsaG.

Figure 5

  1. Ternary complex formation between RsaI, various mRNAs (glcU_2, HG001_00942 and HG001_1242), and RsaG. The 5′ end‐labeled RsaI was incubated with increasing concentrations of the target mRNA alone or in the presence of 50 nM of RsaG. The various complexes are notified on the sides of the autoradiographies.
  2. RsaG does not form stable complexes with glcU_2 and HG001_01242. Binding assays were done in the presence of 5′ end‐labeled RsaG and either cold RsaI (300 nM) or increasing concentrations of glcU_2 and HG001_01242.
  3. Measurements of the half‐lives of RsaI and RsaG in HG001‐∆rsaG or HG001‐∆rsaI mutant strains using Northern blot experiments. The cells were treated with rifampicin at 4 h of growth, and total RNAs were extracted after 2, 4, 8, 15, 30, and 60 min at 37°C in BHI. 5S rRNA was probed to quantify the yield of RNAs in each lane using the same samples, which were however run on two different gels. Calculated half‐lives are shown beneath the autoradiographies and are the average of 2 experiments. The data were normalized to 5S rRNA.

Source data are available online for this figure.