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. 2019 Jan 21;156(4):356–372. doi: 10.1111/imm.13033

Figure 4.

Figure 4

Immunohistochemistry for immature, M1, and M2 macrophages in spleens of rats on standard or zinc‐deficient diet. (a) Representative photomicrographs. Brown spots indicate immunohistochemical staining for CD68 (a marker of immature macrophages), CD163 (a marker of M2 macrophages), and CD169 (a marker of M1 macrophages). Magnification, 400×. (b) Number of positive cells per field. Data represent the mean ± standard error (n = 5 per group). *P < 0·05 versus standard using the Mann–Whitney U‐test.