Table 2.

Sequences and annealing temperatures of primers used in this study

Primers	Base sequence	Annealing temperatures (°C)
18S rRNA	sense: 5′‐CCTGGAAAGGGCTCAACAC‐3′	48a
	anti‐sense: 5′‐GCTCTAGAATTACCACAGTTATCCAA‐3′
IL‐1β	sense: 5′‐ACAAGTGRTATTCTCCATGAGC‐3′ (R is a mixture of A and G)	55b
	anti‐sense: 5′‐CCACTTTGSTCTTGACTTCTAT‐3′ (S is a mixture of C and G)
IL‐4	sense: 5′‐ACCAGACGTCCTTACGGC‐3′	48a
	anti‐sense: 5′‐GCGTGGACTCATTCACGG‐3′
IL‐6	sense: 5′‐GTTGCCTTCTTGGGACTGATGT‐3′	511
	anti‐sense: 5′‐GGTCTGTTGTGGGTGGTATCCT‐3′
IL‐13	sense: 5′‐GCTGTTGCACAGGGAAGTCT‐3′	48a
	anti‐sense: 5′‐GCTGTTGCACAGGGAAGTCT‐3′
TNF‐α	sense: 5′‐CTTATCTACTCCCAGGTTCTCTTCAA‐3′	54c
	anti‐sense: 5′‐GAGACTCCTCCCAGGTACATGG‐3′
MCP‐1	sense: 5′‐CGTGCTGTCTCAGCCAGAT‐3′	48c
	anti‐sense: 5′‐GGATCATCTTGCCAGTGAATG‐3′
MIP‐1α	sense: 5′‐GCGCTCTGGAACGAAGTCT‐3′	48c
	anti‐sense: 5′‐GAATTTGCCGTCCATAGGAG‐3′
IFN‐γ	sense: 5′‐AGGACGGTAACACGAAA‐3′	48a
	anti‐sense: 5′‐CTGTGGGTTGTTCACCTC‐3′

^aRoche universal probe library.

^bTsunoda et al., 2006.

^cKido et al., 2014.