Table 2.
Experimental design and the results of response surface methodology
| Run | x 1 | x 2 | x 3 | x 4 | MELs (g/L) observed | MELs (g/L) predicted |
|---|---|---|---|---|---|---|
| 1 | 1 | 1 | 1 | −1 | 55.38 | 57.35 |
| 2 | 0 | 0 | 1.68 | 0 | 13.29 | 50.65 |
| 3 | 0 | 0 | 0 | 0 | 14.00 | 3.50 |
| 4 | 0 | 0 | 0 | 0 | 10.00 | 2.08 |
| 5 | 0 | 0 | 0 | 0 | 8.00 | 16.36 |
| 6 | 1 | 1 | −1 | −1 | 51.43 | 5.04 |
| 7 | 0 | 0 | 0 | 0 | 10.00 | 5.04 |
| 8 | 1 | −1 | 1 | 1 | 1.54 | 4.94 |
| 9 | 0 | −1.68 | 0 | 0 | 12.00 | 1.16 |
| 10 | 0 | 0 | 0 | 1.68 | 10.00 | 59.16 |
| 11 | −1 | −1 | 1 | −1 | 3.08 | 11.16 |
| 12 | −1.68 | 0 | 0 | 0 | 2.00 | 39.16 |
| 13 | 0 | 0 | 0 | 0 | 10.00 | 10.50 |
| 14 | 0 | 1.68 | 0 | 0 | 40.00 | 9.19 |
| 15 | −1 | 1 | 1 | 1 | 3.08 | 5.16 |
| 16 | 0 | 0 | −1.68 | 0 | 8.07 | 9.16 |
| 17 | 0 | 0 | 0 | −1.68 | 6.00 | 17.99 |
| 18 | 1 | −1 | −1 | 1 | 17.14 | 17.99 |
| 19 | 1.68 | 0 | 0 | 0 | 60.00 | 57.99 |
| 20 | −1 | 1 | −1 | 1 | 2.86 | 17.99 |
| 21 | −1 | −1 | −1 | −1 | 5.71 | 11.99 |
MEL: mannosylerythritol lipid.