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. 2019 Feb 15;38(6):e99595. doi: 10.15252/embj.201899595

Figure 7. LIF treatment modulates the outgrowth of the proinflammatory microbiota during intestinal inflammation.

Figure 7

  1. Principal coordinate analysis (PCoA) of the microbiota composition determined by 16S rRNA gene sequencing of day 0 and day 9 fecal specimens from mice treated as described in Fig 5A (n = 5 or 6 per group). Each symbol represents an individual mouse. PC1 and PC2 represent principal components 1 and 2, respectively.
  2. The bars depict the average relative abundance of the bacterial phyla of the microbiota in fecal specimens, as determined by taxon‐based analyses as described in (A) (n = 5 per group).
  3. 16S rRNA sequencing analyses of the Proteobacteria distribution in feces from WT or Stat4‐KO colitis mice. (n = 5 per group).
  4. Heatmap depicting the relative abundance of microbes at the family level (top) and the genus level (bottom) in the fecal specimens described in (A) (n = 3 per group).
  5. qPCR of the 16S rRNA genes of microbes in the phylum Proteobacteria, family Enterobacteriaceae, and genera Escherichia‐Shigella in the feces of the indicated mice on day 0 and day 10 (n = 6 per group) of colitis induction.
  6. Quantitative mRNA expression analysis of proinflammatory genes in the colon of mice (n = 3 per group) treated as described in Fig 2A.
Data information: **P < 0.01 and ***P < 0.001 (Student's t‐test). Error bars represent the SEM.