Figure 3.

The overexpression of PARP‐2 attenuated the effect of nobiletin on C666‐1 cells. Before being treated with 50 μM nobiletin, C666‐1 cells have been pretreated with PARP‐2 for 1 hr. The groups treated with nobiletin or PARP‐2 only are the positive controls, and the group treated with DMSO is the negative control. After being treated for 48 hr, the cells were applied to perform flow cytometry (A–C) or protein extraction for Western blot of cleaved‐caspase 3 and β‐actin (D). *p < 0.05 vs. the group without treatment, n = 3. ^# p < 0.05 vs. the group treating with nobiletin, n = 3