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. 2017 Aug 31;9(9):398. doi: 10.3390/polym9090398

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Laser confocal scanning microscopy of fluorescein isothiocyanate (FITC) cellular uptake after permeabilization of bacterial membranes. Multidrug-resistant P. aeruginosa cells (9 × 10⁸ CFU/mL) were incubated with 30 μg/mL POGH for 60 min. (a) The original image of POGH-treated cells observed by the ordinary fluorescence mode; (b) the image observed by the mode of Differential Interference Contrast (DIC); (c) the merged image of the corresponding original image and the DIC image; (d,e) the corresponding images in the y–z plane.