(a) Kinetic evaluation of the interaction on the 2D surface, myoglobin at 0.03, 0.06, 0.12, and 0.24 µM, was injected over the surface, respectively; (b) Kinetic evaluation on the 3D carboxymethyl dextran (500 kDa) surface, myoglobin at 0.03, 0.06, 0.12, and 0.24 µM, was injected over the surface, respectively; (c) Kinetic evaluation on the 3D carboxymethyl dextran (2000 kDa) surface, myoglobin at 0.03, 0.06, 0.12, and 0.24 µM, was injected over the surface, respectively. In Figure 5a–c, the measured responses are presented in black lines, the theoretical 1:1 fit generated by the ClampXP software are overlaid in red lines.