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(a) Binding of myoglobin to the immobilized 3D carboxymethyl dextran (2000 kDa) surface and subsequent release of the bound myoglobin via injection of low pH buffer (0.1 M glycine, pH 1.5). (1) binding of myoglobin; (2) regeneration with 0.1 M glycine. The 3D surface was completely regenerated after one injection of the glycine solution for 30 s; (b) the reproducibility of the surface was examined by running 10 repeats of the myoglobin.
