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SDS-PAGE electrophoretogram of E. coli BL21 total proteins under different expression conditions. (A–C) different IPTG concentration (mM); (E,F) different induce time (h); (G–I): different initial cell density (OD600). (A,D,G): GST-GS16F1; (B,E,H): GST-GS16F4; and (C,F,I): GST-GS16F8.
