Figure 7.

Representative example showing a more robust somatodendritic release of VP evoked by NMDAR‐mediated firing when compared to continuous or bursting firing in the same eGFP–VP neurone

A, fluorescence images of sniffer_VP cells (a) in the vicinity of a patched eGFP–VP neurone loaded with Alexa 488 (b). Both images are superimposed in c. B–D, corresponding pseudocolour images showing basal (a) and sniffer_VP Ca²⁺ responses when the patched neurone was stimulated to generate a RBF pattern (Bb), a CF pattern (Cb) or when firing was evoked by focal application of NMDA (10 μm, Db), in the sequence displayed. Plots of sniffer_VP Ca²⁺ responses to each of the stimulation protocols are shown in panels c. Note that in this eGFP–VP neurone, only NMDAR‐evoked firing resulted in robust Ca²⁺ responses in 7 different sniffer_VP cells (arrows, Db). Arrowheads in panels c indicate the stimulation time, and asterisks correspond to the time points of the images shown in panels a and b. Scale bar: 15 μm. [Color figure can be viewed at wileyonlinelibrary.com]