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. Author manuscript; available in PMC: 2019 Mar 15.
Published in final edited form as: Cell Rep. 2018 Aug 7;24(6):1415–1424. doi: 10.1016/j.celrep.2018.07.021

Figure 3. Regulation of mRNAs upon 4 hr of Metabolic Stress Conditions.

Figure 3.

(A) Changes in mRNA and ribosome occupancy levels upon 4-hr deprivation of BCAAs relative to complete medium in transformed (left) and non-transformed (right) cells. Ang, angiogenesis; DN, down-regulated; RO, ribosome occupancy; UP, up-regulated.

(B and C) Venn diagram showing the anota2seq analysis of mRNAs with increased (B) transcriptional and (C) translational activity in all metabolic stresses in transformed versus non-transformed cells.

(D) Venn diagram showing the mRNAs with increased ribosome occupancy in each metabolic stress condition in transformed cells; cytokines are highlighted in red in non-inflammatory GO terms.

(E and F) Shown here: (E) hierarchical and (F) k-means clustering of ribosome occupancy across metabolic conditions.

(G) Correlation between protein synthesis and ribosome occupancy of regulated cytokines among nutrient-deprived conditions.

(H) Ribosome occupancy of all TE-downregulated mRNAs in each metabolic condition; p values are derived from the Wilcoxon test.

See also Figures S2 and S3.