Table 1.

Advantages and disadvantages of the mounting strategy using the sliding rod holder with a coverslip.

Advantages	Disadvantages
Sample sizes of more than 4 mm in diameter	Sample can only be partially rotated.
Allows to image soft and delicate samples (e.g., spinal cord)	Sample can get in contact with the lens
Possibility of precise 3D acquisition	Glue makes the sample opaque
Possibility of tiled imaging