In vitro cytotoxicity assay in PAMAM-FHR-treated cells. (A,B) GBL-14, (E,F) U373-MG, and (I,J) dermal fibroblasts were incubated with different concentrations of the polymers for 24 h (A,E,I) and 48 h (B,F,J). Cytotoxicity was assessed using a WST-1 assay. Results are shown as the mean ± standard deviation, n = 3. (C,D) GBL-14, (G,H) U373-MG, and (K,L) dermal fibroblasts incubated under the same conditions as those used for the WST-1 assay. Cell viability was assessed by the lactate dehydrogenase (LDH) assay. After 24 h (C,G,K) and 48 h (D,H,L). Results are shown as the mean ± standard deviation, n = 3.