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Intracellular localization of PAMAM-FHR. (A) GBL-14 and (B) dermal fibroblasts were incubated with polymer/Alexa Fluor 546 labeled pJDK or pJDK-apoptin complexes for 24 h. Nuclei were stained before confocal microscopy. (C) GBL-14 and (D) dermal fibroblasts were incubated with Alexa Fluor 488-labeled each polymer/pJDK or pJDK-apoptin complexes for 24 h. Lysosomal images were incubated with LysoTracker Red for 15 min before confocal microscopy.
