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. 2019 Mar 14;12:111. doi: 10.1186/s13071-019-3374-2

Table 2.

A list of identified female salivary gland proteins of Ae. aegypti PMD, PMD-R, and UPK-R strains by NanoLC-MS analysis

SNa (MW/pI) Strain Accession numberb Protein description Prot. scorec PSd No. P/ %Ce Database MW/pI Peptide sequence(s) Gene namef
1 (75/5.3) PMD XP_011493320 AAEL017349-PA 65 36 2/3 72.4/5.1 R.LTPEDIER.M AAEL017349-PA
29 K.VADDDKATMEEAIDEK.I + Oxidation (M)
PMD-R XP_011493320 AAEL017349-PA 65 36 2/3 72.4/5.1 R.LTPEDIER.M AAEL017349-PA
29 K.VADDDKATMEEAIDEK.I + Oxidation (M)
UPK-R XP_011493320 AAEL017349-PA 271 66 4/7 72.4/5.1 K.IVITNDQNR.L AAEL017349-PA
71 K.FEELNMDLFR.S + Oxidation (M)
63 K.DVDEIVLVGGSTR.I
75 K.VFAPEEISAMVLGK.M + Oxidation (M)
2 (73/5.6) PMD ABF18332 Heat-shock cognate 70 40 40 1/1 71.4/5.3 K.VEIIANDQGNR.T nag
PMD-R ABF18332 Heat-shock cognate 70 49 49 1/1 71.4/5.3 K.VEIIANDQGNR.T na
UPK-R ABF18332 Heat-shock cognate 70 163 80 2/3 71.4/5.3 K.DAGTISGLNVLR.I na
83 R.FEELNADLFR.S
3 (72/6.2) PMD AAL76020 Putative secreted protein 84 84 1/1 66.6/5.8 K.TASNDCTPFR.L AAEL000748
PMD-R AAL76020 Putative secreted protein 84 84 1/1 66.6/5.8 K.TASNDCTPFR.L AAEL000748
UPK-R AAL76020 Putative secreted protein 130 62 2/4 66.6/5.8 K.DLEGITDELGK.V AAEL000748
67 K.DIATLEQNVNTLHK.D
4 (69/8.5) PMD AAC37218 Apyrase 231 65 3/6 63.2/8.5 R.TGPLDSDVFK.N APY
82 K.VTLSNAVEAVR.R
84 K.VEAIGSTVVGETK.I
PMD-R AAC37218 Apyrase 449 66 6/12 63.2/8.5 R.TGPLDSDVFK.N APY
61 R.TNCLQVSGLR.I
76 K.VTLSNAVEAVR.R
73 K.VEAIGSTVVGETK.I
96 K.KIEVMDYTNPK.S + Oxidation (M)
80 K.DKVEGPYPTIVESK.N
UPK-R AAC37218 Apyrase 231 81 3/5 63.2/8.5 K.IGIIGVLYDK.T APY
72 R.IVIDISKPIR.S
78 K.VTLSNAVEAVR.R
5 (68/8.9) PMD AAC37218 Apyrase 137 59 2/4 63.2/8.5 R.TNCLQVSGLR.I APY
75 K.VEAIGSTVVGETK.I
PMD-R AAC37218 Apyrase 531 66 7/12 63.2/8.5 R.TGPLDSDVFK.N APY
71 K.IGIIGVLYDK.T
69 R.TNCLQVSGLR.I
87 K.VTLSNAVEAVR.R
68 R.RTGPLDSDVFK.N
79 K.VEAIGSTVVGETK.I
93 K.DKVEGPYPTIVESK.N
UPK-R AAC37218 Apyrase 641 61 9/17 63.2/8.5 R.TGPLDSDVFK.N APY
81 K.IGIIGVLYDK.T
64 R.TNCLQVSGLR.I
70 R.IVIDISKPIR.S
79 K.VTLSNAVEAVR.R
59 K.GLAPYLAELNK.E
60 K.VEAIGSTVVGETK.I
80 K.KIEVMDYTNPK.S + Oxidation (M)
63 K.DKVEGPYPTIVESK.N
6 (68/9.5) PMD XP_001651903 AAEL006333-PA 139 80 2/3 63.9/8.7 R.ISLAAGAITR.G AAEL006333
61 K.LELSNDCR.L
PMD-R XP_001651903 AAEL006333-PA 139 80 2/3 63.9/8.7 R.ISLAAGAITR.G AAEL006333
61 K.LELSNDCR.L
UPK-R ABF18486 5’-nucleotidase 96 20 3/6 61.8/8.9 R.VTVLGSTIK.K AAEL006333
38 K.VGESKLELSNDCR.L
39 R.GQILTALPFNSNANR.V
7 (66/7.2) PMD XP_001651237 AAEL005672-PA 593 37 11/23 59.9/6.4 R.LKLEEMVK.G + Oxidation (M) AAEL005672
48 R.HIFEVLDR.F
45 R.FATDDEFLK.I
35 K.SNHPEFIGAK.F
51 K.EWSLVSEIR.Q
51 R.SLLEFAPALLK.L
52 K.GDFGVSHGPQFK.C
70 R.GVLPDVYDLDGK.I
87 K.TDQNLIDAVLLGSK.R
62 K.QFYDDHVQYLEFR.G
54 K.QLALNSIEYSAMNSEEK.T + Oxidation (M)
PMD-R AAL76033 Putative adenosine deaminase 100 22 3/4 60.6/6.5 K.FIYAPGR.F AAEL005672
36 K.SSHPEFIGAK.F
45 R.FATDDEFLK.I
UPK-R AAL76033 Putative adenosine deaminase 219 70 3/7 60.6/6.5 R.GVLPDVYDLDGK.I AAEL005672
87 K.TDQNLIDAVLLGSK.R
62 K.QFYDDHVQYLEFR.G
8 (62/5.1) PMD XP_001649774 AAEL000641-PA 163 77 2/4 56.3/5.0 K.MDATANELEHTK.I + Oxidation (M) AAEL000641
86 K.VDATEEQELSEK.H
PMD-R XP_001649774 AAEL000641-PA 47 47 1/2 56.3/5.0 R.NGTPIEYTGGR.E AAEL000641
UPK-R XP_001649774 AAEL000641-PA 82 82 1/2 56.3/5.0 K.VDATEEQELSEK.H AAEL000641
9 (59/6.6) PMD ABF18028 Salivary anti-FXa serpin 121 59 2/5 48.3/6.1 R.MGQVINDALK.N + Oxidation (M) SRPN23
62 K.TPPEAAMGLEDK.Q + Oxidation (M)
PMD-R ABF18028 Salivary anti-FXa serpin 58 58 1/2 48.3/6.1 R.TAMAVAIGSEK.V + Oxidation (M) SRPN23
UPK-R ABF18028 Salivary anti- FXa serpin 307 59 4/11 48.3/6.1 R.TAMAVAIGSEK.V + Oxidation (M) SRPN23
77 R.MGQVINDALK.N + Oxidation (M)
106 R.IPQFGLQTTVPGR.Q
64 K.VFEQGQDVALGEIVQK.M
10 (49/5.1) PMD AAL76010 Putative purine hydrolase 48 48 1/2 38.2/5.0 R.QMVEDLNR.- + Oxidation (M) AAEL006485
PMD-R AAL76010 Putative purine hydrolase 76 42 2/4 38.2/5.0 R.LITPAPR.R AAEL006485
34 R.QMVEDLNR.- + Oxidation (M)
UPK-R AAL76010 Putative purine hydrolase 73 73 1/3 38.2/5.0 K.SEIAGIYILGGNR.N AAEL006485
11 (48/6.0) PMD ABF18025 Angiopoietin-like protein variant, partial 68 22 2/6 33.5/5.7 K.FVVGPEEQR.Y AAEL000749
45 K.FSDVSNTPLK.L
PMD-R AAL76032 Angiopoietin-like protein 68 22 2/6 33.5/5.7 K.FVVGPEEQR.Y AAEL000749
45 K.FSDVSNTPLK.L
UPK-R ABF18025 Angiopoietin-like protein variant, partial 153 37 4/15 33.5/5.7 K.FVVGPEEQR.Y AAEL000749
32 K.FSDVSNTPLK.L
35 K.SSGLTTIPIGSEPR.V
49 R.FTQMFSQQFYR.H + Oxidation (M)
12 (45/5.8) PMD ABF18018 Putative 34 kDa secreted protein 64 64 1/3 36.4/5.2 K.LQEEIEEQTK.Q AAEL003601
PMD-R ABF18018 Putative 34 kDa secreted protein 64 64 1/3 36.4/5.2 K.LQEEIEEQTK.Q AAEL003601
UPK-R ABF18018 Putative 34 kDa secreted protein 132 65 2/6 36.4/5.2 K.SVATEIVQMR.D + Oxidation (M) AAEL003601
68 K.QNFEDQVNQIVK.S
13 (46/5.7) PMD AAL76018 Putative 34 kDa secreted protein 54 54 1/4 36.4/5.2 R.DSAISTDQVDQLK.Q AAEL003601
PMD-R AAL76018 Putative 34 kDa secreted protein 155 74 2/7 36.4/5.2 R.DSAISTDQVDQLK.Q AAEL003601
81 K.QNFEDQVNQIVK.S
UPK-R AAL76018 Putative 34 kDa secreted protein 176 40 4/12 36.4/5.2 R.IYLGALR.F AAEL003601
54 K.SVATEIVQMR.D + Oxidation (M)
51 K.VTELEQQIAK.Q
35 K.QNFEDQVNQIVK.S
14 (43/6.2) PMD ABF18017 Putative 34 kDa family secreted salivary protein 98 49 2/6 36.0/6.1 K.LQEEIEEQTK.Q AAEL003600
53 K.QCNLSEDDLTK.L
PMD-R ABF18017 Putative 34 kDa family secreted salivary protein 206 73 3/12 36.0/6.1 K.QCNLSEDDLTK.L AAEL003600
75 K.TQGVSNMEVQLLR.E + Oxidation (M)
60 R.QANQDTSKAEGEMVEK.I + Oxidation (M)
UPK-R ABF18017 Putative 34 kDa family secreted salivary protein 256 66 3/8 36.0/6.1 K.VLNTILDQVNK.L AAEL003600
86 R.KVLNTILDQVNK.L
106 K.TQGVSNMEVQLLR.E
15 (40/9.5) PMD AAL16049 Long form D7Bclu1 salivary protein 450 64 6/18 39.2/9.4 K.NELDVDEIAR.D AAEL006417
93 R.SKDYFAALTGK.L
74 K.SSSCSEVFEAFK.K
75 R.KQVDDIDKIQCS.-
63 K.SSSCSEVFEAFKK.V
84 K.LEPSDDQATQCYTK.C
PMD-R XP_001657778 AAEL006417-PA 614 58 8/29 39.2/9.4 K.IGFYEPGEKR.F AAEL006417
91 R.SKDYFAALTGK.L
68 K.YQMGSGIVFER.H + Oxidation (M)
83 K.SSSCSEVFEAFK.K
72 R.KQVDDIDKIQCS.-
68 K.VTNDFKEAFDYR.E
88 K.LEPSDDQATQCYTK.C
88 R.YMTSKNELDVDEIAR.D + Oxidation (M)
UPK-R AAL16049 Long form D7Bclu1 salivary protein 385 63 5/14 39.2/9.4 K.AALQNWLGWK.L AAEL006417
82 R.SKDYFAALTGK.L
87 K.SSSCSEVFEAFK.K
60 K.SSSCSEVFEAFKK.V
94 K.LEPSDDQATQCYTK.C
16 (39/9.5) PMD AAA29347 D7 protein 437 72 6/21 37.6/8.4 K.SKVEAYANAVK.Q D7
71 K.FDASVIQEQFK.A
58 R.QYTVLDDALFK.E
75 K.DNQLDVEEVKR.D
85 K.CLVESSVKDDFK.E
83 R.CMEDNLEDGANR.L + Oxidation (M)
PMD-R AAA29347 D7 protein 363 66 5/17 37.6/8.4 K.SKVEAYANAVK.Q D7
60 K.DDFKEAFDYR.E
69 K.FDASVIQEQFK.A
72 K.QSYFEFCENK.Y
102 K.QLPSTNNDCAAVFK.A
UPK-R AAA29347 D7 protein 390 61 6/21 37.6/8.4 R.TGLYDPVAQK.F D7
68 K.SKVEAYANAVK.Q
71 K.FDASVIQEQFK.A
67 R.QYTVLDDALFK.E
63 K.DNQLDVEEVKR.D
70 K.QLPSTNNDCAAVFK.A
17 (40/8.9) PMD AAA29348 D7 protein 153 55 3/9 37.5/8.5 R.TGLYDPVAQK.F D7
75 K.NNELDAEEVKR.D
23 K.KSYFEFCENK.Y
PMD-R AAA29348 D7 protein 191 65 3/10 37.5/8.5 K.FDASVIQEQFK.A D7
71 R.QYTVLDDALFK.E
59 K.NNELDAEEVKR.D
UPK-R AAA29347 D7 protein 223 67 3/10 37.6/8.4 R.QYTVLDDALFK.E D7
84 K.DNQLDVEEVKR.D
76 K.CLVESSVKDDFK.E
18 (42/8.9) PMD AAA29348 D7 protein 75 75 1/3 37.5/8.5 K.NNELDAEEVKR.D D7
PMD-R AAA29348 D7 protein 208 63 3/9 37.5/8.5 K.DDFKEAFDYR.E D7
73 K.FDASVIQEQFK.A
74 K.KSYFEFCENK.Y
UPK-R AAA29348 D7 protein 211 74 3/9 37.5/8.5 K.FDASVIQEQFK.A D7
67 R.QYTVLDDALFK.E
74 K.KSYFEFCENK.Y
19 (33/9.5) PMD XP_001651000 AAEL000793-PA 199 87 2/16 29.6/9.1 R.MPTLVWDDELAHIASFNAR.K + Oxidation (M) AAEL000793
112 R.RGPHVACNAPTQFGSACGQEPK.F
PMD-R AAL76012 Putative secreted protein 199 87 2/16 29.6/9.1 R.MPTLVWDDELAHIASFNAR.K + Oxidation (M) AAEL000793
112 R.RGPHVACNAPTQFGSACGQEPK.F
UPK-R AAL76009 Putative secreted protein 38 38 1/3 30.2/9.0 K.DYCSSVFR.K AAEL003053
20 (33/4.6) PMD AAX54871 Putative 19.6 kDa secreted protein 31 31 1/4 21.8/4.8 K.VIEQLDQIK.V AAEL010228
PMD-R XP_001660690 AAEL010228-PA 166 62 2/12 21.8/4.8 K.DSNAYQCSQDR.S AAEL010228
104 R.NPIVDVIGSAGDFSK.I
UPK-R XP_001660690 AAEL010228-PA 173 66 2/12 21.8/4.8 K.DSNAYQCSQDR.S AAEL010228
107 R.NPIVDVIGSAGDFSK.I
21 (31/4.1) PMD ABF18125 30 kDa salivary gland allergen variant 3 124 64 2/6 27.9/4.2 R.VPVVEAIGR.I AAEL010235
61 K.NDPADTYR.Q
PMD-R ABF18125 30 kDa salivary gland allergen variant 3 452 64 6/18 27.9/4.2 R.VPVVEAIGR.I AAEL010235
61 R.QVVALLDKDTK.V
86 R.SALNNDLQSEVR.V
75 K.SEYQCSEDSFAAAK.S
80 K.KSEYQCSEDSFAAAK.S
88 K.KKSEYQCSEDSFAAAK.S
UPK-R ABF18125 30 kDa salivary gland allergen variant 3 301 61 4/17 27.9/4.2 K.NDPADTYR.Q AAEL010235
62 R.QVVALLDKDTK.V
107 R.SALNNDLQSEVR.V
71 K.SEYQCSEDSFAAAK.S
22 (15/9.5) PMD AAL16050 Short form D7Cclu23 salivary protein 32 32 1/6 18.2/9.0 R.FFTEKPFCK.- AAEL006423
PMD-R AAL16050 Short form D7Cclu23 salivary protein 163 47 4/22 18.2/9.1 K.INGWAYFR.C AAEL006423
46 R.FFTEKPFCK.-
54 R.NFLCHTANFK.L
16 K.TEMGDKINGWAYFR.C + Oxidation (M)
UPK-R AAL16050 Short form D7Cclu23 salivary protein 32 32 1/6 18.2/9.1 R.FFTEKPFCK.- AAEL006423
23 (15/5.6) PMD AAL76029 Putative C-type lectin 33 33 1/6 18.1/5.3 K.QQQVEELAQR.V CTL16
PMD-R XP_001647974 AAEL000533-PA 87 46 2/12 18.1/5.3 R.LAVLDSEQK.Q CTL16
40 K.QQQVEELAQR.V
UPK-R AAL76029 Putative C-type lectin 93 51 2/12 18.1/5.3 R.LAVLDSEEK.Q CTL16
42 K.QQQVEELAQR.V
24 (15/5.9) PMD AAL76035 Putative 16.9 kDa secreted protein 135 23 3/20 17.4/5.5 K.TELDEVTK.E SG14.5sp
32 R.NLQVDGNMPK.V + Oxidation (M)
80 K.VAQDMVPYGFNIK.T + Oxidation (M)
PMD-R ABF18031 Short salivary D7 protein, partial 80 80 1/8 17.5/5.7 K.VAQDMVPYGFNIK.T + Oxidation (M) na
UPK-R ABF18031 Short salivary D7 protein, partial 75 75 1/8 17.5/5.7 K.VAQDMVPYGFNIK.T + Oxidation (M) na
A1 (20/8.6) PMD ABF18082 Short D7 protein 44 44 1/5 18.6/8.2 R.QNQIEELNK.L SG16.2sp
A2 (56/6.2) PMD-R ABF18028 Salivary anti-FXa serpin 229 70 3/8 48.3/6.1 R.TAMAVAIGSEK.V + Oxidation (M) SRPN23
69 K.MEMSIAADGEK.Q + Oxidation (M)
90 K.TPPEAAMGLEDK.Q + Oxidation (M)
UPK-R ABF18028 Salivary anti-FXa serpin 95 95 1/3 48.3/6.1 R.IPQFGLQTTVPGR.Q SRPN23
A4 (59/6.2) UPK-R ABF18509 Salivary serpin 235 62 3/8 48.2/6.3 R.MGQVINDALK.N + Oxidation (M) SRPN23
68 K.TPPEAGMGLEDK.Q + Oxidation (M)
105 R.IPQFGLQTTVPGR.Q

aSpot number refers to those shown in Fig. 2 /(Observed MW/pI)

bAccession number of the best hit of proteins from Ae. aegypti, available at http://www.ncbi.nlm.nih.gov

cProt. Score = Protein score, Mowse score ≥ 30

dPS = Peptide score

eNo. P/%C = Number of peptide/% coverage

fName of the gene that codes for the protein sequences, available at http://www.uniprot.org/uploadlists

gna, not available